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eclipse ti2000 epifluorescence microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti2000 epifluorescence microscope
    Eclipse Ti2000 Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti2000+epifluorescence+microscope/10__1002_slash_slct__202403837-274-2-1
    Average 90 stars, based on 1 article reviews
    eclipse ti2000 epifluorescence microscope - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Structure-Activity Relationship Studies of the Aryl Acetamide Triazolopyridazines against Cryptosporidium Reveals Remarkable Role of Fluorine.
    Article Snippet: Our previous work identified compound 1 (SLU-2633) as a potent lead compound toward the identification of a novel treatment for cryptosporidiosis, caused by the parasite Cryptosporidium (EC50 = 0.17 μM).. While this compound is potent and orally efficacious, the mechanism of action and biological target(s) of this series are currently unknown.. In this study, we synthesized 70 compounds to develop phenotypic structure−activity relationships around the aryl “tail” group.

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: .. A Nikon Eclipse Ti2000 epifluorescence microscope with an automated stage was programmed using NIS-Elements Advanced Research software (Nikon, USA) to focus on the center of each well and take a 3 × 3 or 6 × 6 composite image using an EXi blue fluorescence microscopy camera (QImaging, Canada) with a 20 × objective (NA= 0.45). ..

    Article Title: Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 in Cryptosporidium parvum and HCT-8 Host Cells
    Article Snippet: .. A Nikon Eclipse Ti2000 epifluorescence microscope programmed using NIS-Elements Advanced Research software ( https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research ; 1 January 2024; Nikon, Toko, Japan) was used take a 3 × 3 composite image using an EXi blue fluorescence microscopy camera (QImaging, Surrey, BC, Canada) and a 20 × objective (NA = 0.45). ..

    Article Title: Cryptosporidium life cycle small molecule probing implicates translational repression and an Apetala 2 transcription factor in macrogamont differentiation.
    Article Snippet: .. The culture plates were imaged with Nikon Eclipse Ti2000 epifluorescence microscope equipped with automated stage movement, using an objective with 20X magnification and 0.45 numerical aperture (NA). ..

    Article Title: Cryptosporidium Life Cycle Small Molecule Probing Implicates Translational Repression And An Apetala 2 Transcription Factor In Sexual Differentiation
    Article Snippet: .. The culture plates were imaged with Nikon Eclipse Ti2000 epifluorescence microscope equipped with automated stage movement, using an objective with 20X magnification and 0.45 numerical aperture (NA). ..

    Article Title: Synthesis of β‐fluoropyrrolidines Designed to Reduce hERG Affinity in a Class of Antiparasitic Compounds
    Article Snippet: Malaria is a parasitic disease caused by the parasite Plasmodium which results in hundreds of thousands of deaths per year.. The need for new drugs for the treatment of this disease is imperative as new resistant strains of Plasmodium emerge.. Previously, our group reported two related series of pyrrolidinebased antimalarial compounds.

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: Cells were then co-stained with a 1:500 dilution (4 μg/mL) of Alexa Fluor 568 goat anti-mouse IgG (Invitrogen, catalog# A-11004) and 1.33 μg/mL fluorescein-labeled Vicia villosa lectin (Vector Laboratories, catalog# FL-1231) diluted in blocking buffer for 1 h at 37 °C. .. Stains were then removed and 0.09 mM Hoechst 33258 (AnaSpec, catalog# AS-83219) also diluted in blocking buffer was added for another 15 min at 37 °C, followed by 2 PBS washes and imaging on a Nikon Eclipse Ti2000 epifluorescence microscope run with NIS-Elements Advanced Research software (Nikon, USA) and equipped with an EXi blue fluorescent microscopy camera (QImaging, Canada). ..

    Fluorescence:

    Article Title: Structure-Activity Relationship Studies of the Aryl Acetamide Triazolopyridazines against Cryptosporidium Reveals Remarkable Role of Fluorine.
    Article Snippet: Our previous work identified compound 1 (SLU-2633) as a potent lead compound toward the identification of a novel treatment for cryptosporidiosis, caused by the parasite Cryptosporidium (EC50 = 0.17 μM).. While this compound is potent and orally efficacious, the mechanism of action and biological target(s) of this series are currently unknown.. In this study, we synthesized 70 compounds to develop phenotypic structure−activity relationships around the aryl “tail” group.

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: .. A Nikon Eclipse Ti2000 epifluorescence microscope with an automated stage was programmed using NIS-Elements Advanced Research software (Nikon, USA) to focus on the center of each well and take a 3 × 3 or 6 × 6 composite image using an EXi blue fluorescence microscopy camera (QImaging, Canada) with a 20 × objective (NA= 0.45). ..

    Article Title: Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 in Cryptosporidium parvum and HCT-8 Host Cells
    Article Snippet: .. A Nikon Eclipse Ti2000 epifluorescence microscope programmed using NIS-Elements Advanced Research software ( https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research ; 1 January 2024; Nikon, Toko, Japan) was used take a 3 × 3 composite image using an EXi blue fluorescence microscopy camera (QImaging, Surrey, BC, Canada) and a 20 × objective (NA = 0.45). ..

    Article Title: Synthesis of β‐fluoropyrrolidines Designed to Reduce hERG Affinity in a Class of Antiparasitic Compounds
    Article Snippet: Malaria is a parasitic disease caused by the parasite Plasmodium which results in hundreds of thousands of deaths per year.. The need for new drugs for the treatment of this disease is imperative as new resistant strains of Plasmodium emerge.. Previously, our group reported two related series of pyrrolidinebased antimalarial compounds.

    Software:

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: .. A Nikon Eclipse Ti2000 epifluorescence microscope with an automated stage was programmed using NIS-Elements Advanced Research software (Nikon, USA) to focus on the center of each well and take a 3 × 3 or 6 × 6 composite image using an EXi blue fluorescence microscopy camera (QImaging, Canada) with a 20 × objective (NA= 0.45). ..

    Article Title: Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 in Cryptosporidium parvum and HCT-8 Host Cells
    Article Snippet: .. A Nikon Eclipse Ti2000 epifluorescence microscope programmed using NIS-Elements Advanced Research software ( https://www.microscope.healthcare.nikon.com/products/software/nis-elements/nis-elements-advanced-research ; 1 January 2024; Nikon, Toko, Japan) was used take a 3 × 3 composite image using an EXi blue fluorescence microscopy camera (QImaging, Surrey, BC, Canada) and a 20 × objective (NA = 0.45). ..

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: Cells were then co-stained with a 1:500 dilution (4 μg/mL) of Alexa Fluor 568 goat anti-mouse IgG (Invitrogen, catalog# A-11004) and 1.33 μg/mL fluorescein-labeled Vicia villosa lectin (Vector Laboratories, catalog# FL-1231) diluted in blocking buffer for 1 h at 37 °C. .. Stains were then removed and 0.09 mM Hoechst 33258 (AnaSpec, catalog# AS-83219) also diluted in blocking buffer was added for another 15 min at 37 °C, followed by 2 PBS washes and imaging on a Nikon Eclipse Ti2000 epifluorescence microscope run with NIS-Elements Advanced Research software (Nikon, USA) and equipped with an EXi blue fluorescent microscopy camera (QImaging, Canada). ..

    Blocking Assay:

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: Cells were then co-stained with a 1:500 dilution (4 μg/mL) of Alexa Fluor 568 goat anti-mouse IgG (Invitrogen, catalog# A-11004) and 1.33 μg/mL fluorescein-labeled Vicia villosa lectin (Vector Laboratories, catalog# FL-1231) diluted in blocking buffer for 1 h at 37 °C. .. Stains were then removed and 0.09 mM Hoechst 33258 (AnaSpec, catalog# AS-83219) also diluted in blocking buffer was added for another 15 min at 37 °C, followed by 2 PBS washes and imaging on a Nikon Eclipse Ti2000 epifluorescence microscope run with NIS-Elements Advanced Research software (Nikon, USA) and equipped with an EXi blue fluorescent microscopy camera (QImaging, Canada). ..

    Imaging:

    Article Title: A suite of phenotypic assays to ensure pipeline diversity when prioritizing drug-like Cryptosporidium growth inhibitors.
    Article Snippet: Cells were then co-stained with a 1:500 dilution (4 μg/mL) of Alexa Fluor 568 goat anti-mouse IgG (Invitrogen, catalog# A-11004) and 1.33 μg/mL fluorescein-labeled Vicia villosa lectin (Vector Laboratories, catalog# FL-1231) diluted in blocking buffer for 1 h at 37 °C. .. Stains were then removed and 0.09 mM Hoechst 33258 (AnaSpec, catalog# AS-83219) also diluted in blocking buffer was added for another 15 min at 37 °C, followed by 2 PBS washes and imaging on a Nikon Eclipse Ti2000 epifluorescence microscope run with NIS-Elements Advanced Research software (Nikon, USA) and equipped with an EXi blue fluorescent microscopy camera (QImaging, Canada). ..



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